Figure 1.
Cytokine production in M(IFN-γ/LPS) pretreated or not with lupeol before stimulation with 7-keto-cholesterol. Primary M(IFN-γ/LPS) macrophages (7 × 105 cells per mL) were stimulated or not with lupeol at the different concentrations for 1 hour and further stimulated with 7KC in complete medium. Supernatants were collected after 20 hours to measure cytokines by specific ELISA experiments. Results are expressed as mean value ± SD of 3 independent experiments. P values were tested by one-way ANOVA. ∗† lupeol 10 μM vs. lupeol 25 μM; ‡ 7KC-treated group vs. 7KC+lupeol 25 μM.