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. 2020 Sep 27;2020:1232816. doi: 10.1155/2020/1232816

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Copyright © 2020 Sarmistha Saha et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Flow cytometric analysis of surface marker expressions on M_(IFN-γ/LPS) macrophages. Lupeol skews primary M_(IFN-γ/LPS) macrophage phenotype towards an anti-inflammatory phenotype and prevents 7-keto-cholesterol (7KC) induced changes in M_(IFN-γ/LPS) macrophages. M_(IFN-γ/LPS) primary macrophages were stimulated or not with lupeol at 25 μM for 1 hour and further stimulated with 7KC and then analyzed for HLA-DR and CD36 expressions by flow cytometry. (a) The results of one representative experiment of three are shown. The number in the histograms shows the percentages of positive cells (%) and the mean fluorescence intensity (MFI). (b) Flow cytometric analysis of surface marker expression on M_(IFN-γ/LPS) macrophages. Results are expressed as % and MFI (mean ± SD; n = 3). P values were calculated by one-way ANOVA with a Tukey post hoc test.