FIGURE 1.

Established ccRCC ALI PDOs. Resected tumor tissue was cultivated by mechanistic fragmentation and subsequent uptake in a collagen I solution. Ultimately, the fragmented kidney tissue was plated in a collagen-coated insert and placed in a cell culture dish (A). 42 kidney tissue types were taken into culture, namely 26 ccRCCs, 5 pRCCs, 8 urothelial carcinomas, and 3 oncocytomas (B). Clinical data, including sex, T stage and grading, of the cultured ccRCC ALI PDOs (C). Light microscopy pictures of cultivated kidney PDOs taken at 5x magnification (D).