Fig. 2.

Polymerase β deficiency results in CI impairment in hippocampal synaptosomes and upregulated CII activity in female non-synaptic hippocampal mitochondria. a–d Maximal electron flow through complex I under malate (2 mM) and pyruvate (10 mM) substrates, complex II with succinate (10 mM) and rotenone (2 μM), and complex IV with TMPD (100 μM) + Ascorbate (10 mM) in male (blue; left) and female (red; right) hippocampal synaptic (HSYN) and non-synaptic (HNS) brain mitochondria n = 4–6/group/One-way ANOVA with Benjamini, Krieger and Yekutieli post hoc test for multiple comparisons p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. vs. WT, NS = non-significant. e–j Western blot of cortical synaptic mitochondrial oxidative phosphorylation proteins, two-way ANOVA with Benjamini, Krieger and Yekutieli post hoc test for multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. sex-matched WT, unless indicated by brackets. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, significant effect of sex, n = 3/group