Table 1. Arsenic stocks and serial dilutions.

This table explains the calculations used to create the As stock solutions and working stock solutions for treatment groups.

1000 μM As Stock Calculations	Arsenic Working Stock Concentrations	M1V1=M2V2	Volume of Previous Arsenic Solution	Volume of Media	Total Volume of Arsenic Working Stock
NaAsO2 molecular weight: 129.9 g/mol	10 μM As	(x mL)(1,000 μM) = (30 mL)(10 μM) x = 0.3 mL	0.3 = 300 μL of 1mM As solution	29.7 mL media	30
.03 L x .001 mol/L x 130 g/mol = 3.9 mg of Arsenic	1.0 μM As	(x mL)(10 μM) = (30 mL)(1 μM) x = 3 mL	3 mL of 10 μM As solution	27 mL of media	30
Dilute 3.9 mg of sodium arsenite into 30 mL media	0.1 μM As	(x mL)(1 μM) = (30 mL)(0.1 μM) x = 3 mL	3 mL of 1 μM As	27 mL of media	30
	0.01 μM As	(x mL)(0.1 μM) = (30 mL)(0.01 μM) x = 3 mL	3 mL of 0.1 μM As	27 mL of media	30