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. Author manuscript; available in PMC: 2020 Oct 6.
Published in final edited form as: J Vis Exp. 2019 Feb 23;(144):10.3791/58838. doi: 10.3791/58838

Table of Materials

Name Company Catalog Number Comments
Class II A/B3 Biological Safety Cabinet Forma Scientific 15201-816
Nitrile Gloves Kimberly-Clark 55082
Water Bath Precision Model 182
Inverted Microscope Leica Q080318
CPR Centrifuge Beckman 349702
Analytical Scale Ohaus I093 1125062111P
Weighting paper VWR H351125781212A
Biohazard bags Fisherbrand 01-830A
Water Jacketed CO2 Incubator Thermo Scientific 309739-31053
Hanks Balanced Salt Solution Gibco 14175-095
Dulbecco’s Modified Eagle Medium Gibco 10566-016
Fetal Bovine Serum Gibco 26140-079
Automatic Pipettor Drummond 140685
p200 Pipette Gilson
p20 Pipette Gilson
Denominator Fisher Scientific D59707
Trypan Blue Stain Gibco 15250-061
T75 tissue flask Corning 430725U
15 mL Conical Tube Fisherbrand 05-539-5
50 mL Conical Tube VWR 21008-178
5 mL Pipette Fisherbrand 13-678-11D
10 mL Pipette Fisherbrand 13-678-11E
Tissue Marker Securline 92167
Wipes Kintech 34155
70% Ethanol Fisher 63-67-0
Non-Filter Pipet Tips Fisherbrand 16160210
Bleach Great Value 220-04
96-well Falcon 353915
Crovial Rach Nalgene 5030-0505
Hemacytometer Gizmo Supply B00SCOGY56
TrypLE Express Gibco 12605-028
Conical Tube Rack n/a n/a
12-well plate Corning 3598
Waste Beaker n/a n/a
1 mL Pipette Fisherbrand 13-678-11B
Straight Edge Ruler n/a n/a
Human neonatal dermal fibroblasts (hDFn) lot: 1734, 2909 106-05n
Rstudio Statistical Software ver: 3.5.1
ImageJ NIH ver: 1.8.0_112