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. 2020 Oct 6;15(10):e0240156. doi: 10.1371/journal.pone.0240156

Fig 8. Cytotoxic activities of metabolites extracted from AuNPs.

Fig 8

NPs solution was centrifuged and the NPs were collected and washed by PBS two times prior to extraction using methanol and sonication for 15 min. The extracted metabolites were tested on F180 and MCF7 cells using MTT assay. Mammalian cells were seeded in 96-well plate until confluency followed by incubation with the samples overnight prior to MTT assay. The data were analyzed using two-way ANOVA and statistical significance was calculated with Bonferroni’s multiple comparisons test and significance levels were indicated by asterisks (*, P<0.05; **, P<0.01: ***, P<0.001; ****, P<0.0001). The data display the mean of the percentage of the survival rate of mammalian cells ± SEM of 5 replicas.