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. 2020 Oct 2;12:9577–9587. doi: 10.2147/CMAR.S272309

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© 2020 Lu et al.

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CircNFIX acted as a sponge of miR-212-3p. (A) MiR-212-3p expression by qRT-PCR in 55 pairs of NSCLC tissues and adjacent non-tumor tissues. (B) The correlation between miR-212-3p expression and circNFIX level in NSCLC tissues using the Spearman test. (C) Schematic of the complementary sequence for miR-212-3p within circNFIX identified by starBase v.2 software and the mutation of the seed region. (D) Relative luciferase activity in A549 and H1299 cells cotransfected with circNFIX-WT or circNFIX-MUT and miR-212-3p mimic or miR-NC mimic. (E) The enrichment level of circNFIX by Bio-miR-212-3p or Bio-NC in both A549 and H1299 cells. (F) MiR-212-3p expression by qRT-PCR in A549 and H1299 cells transduced with sh-NC, sh-circ#1.

Notes: **P < 0.01 or ***P < 0.001