Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 2;12:9435–9448. doi: 10.2147/CMAR.S272478

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Shi et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

Hsa_circ_0084927 served as a sponge for miR-634, which targeted TPD52 in CC cells. (A and B) Schematic diagrams exhibiting the potential binding sites of hsa_circ_0084927 or TPD52 and miR-634. (C-F) Dual-luciferase reporter assay was performed to analyze the luciferase activity in Hela and SiHa cells cotransfected with NC mimics or miR-634 mimics and luciferase vectors containing hsa_circ_0084927, mut-hsa_circ_0084927, TPD52 3ʹUTR, or mut-TPD52 3ʹUTR. (G and H) RIP assay was performed to determine whether hsa_circ_0084927 could bind miR-634 in Hela and SiHa cells. (I-K) The levels of miR-634, TPD52 mRNA, and TPD52 protein in 46 paired CC tissues and adjacent non-tumor tissues were analyzed by qRT-PCR or Western blotting. (L-N) The correlation among hsa_circ_0084927, miR-634, and TPD52 mRNA were assessed with Pearson’s correlation analysis. *P < 0.05.