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. 2020 Oct 2;12:9435–9448. doi: 10.2147/CMAR.S272478

Figure 5.

Figure 5

MiR-634 exerted its role through targeting TPD52 in CC cells. (A-N) Hela and SiHa cells were transfected with NC mimics, miR-634 mimics, miR-634 mimics+pcDNA, or miR-634 mimics+TPD52. (A-D) QRT-PCR and Western blotting were conducted to analyze the levels of TPD52 mRNA and protein in Hela and SiHa cells. (E-L) CCK-8, plate clone, flow cytometry, and transwell assays were executed to evaluate the proliferation, colony formation, cell cycle progression, apoptosis, migration, or invasion of Hela and SiHa cells. (M and N) Western blotting was performed to assess the levels of cyclin D1, c-caspase 3, MMP-2, and MMP-9 in Hela and SiHa cells. *P < 0.05.