Skip to main content
. 2020 Oct 2;15:7315–7327. doi: 10.2147/IJN.S262052

Figure 6.

Figure 6

Antifibrotic effect in vivo. The levels of the (A) PPARγ, (B) TGF-β1, (C) α-SMA and (D) Collagen I mRNAs were detected using fluorogenic quantitative RT-PCR. (E) The levels of the PPARγ, TGF-β1, α-SMA and Collagen I proteins were determined using Western blot analysis. (F) Representative photomicrographs showing immunohistochemical staining for TGF-β1, α-SMA and Collagen I and Masson’s trichrome staining in the kidneys (400×). Quantitative evaluation of immunohistochemical staining for (G) TGF-β1, (H) α-SMA and (I) Collagen I. (J) Quantitative analysis of the blue-stained area by Masson’s trichrome staining (n=5, ##p<0.01 and #p<0.05 vs the sham group, *p < 0.05 vs the UUO group).