Skip to main content
. 2020 Oct 2;12:9563–9575. doi: 10.2147/CMAR.S262007

Figure 4.

Figure 4

Silencing circCSNK1G1 blocked the development of CRC in vitro by increasing miR-455-3p. HCT116 and SW620 cells were introduced with si-circCSNK1G1, si-NC, si-circCSNK1G1+anti-miR-455-3p or si-circCSNK1G1+anti-miR-NC. (A and B) The expression of miR-455-3p in these transfected cells was examined by qRT-PCR. (C–E) Cell proliferation in these transfected cells was monitored by MTT assay and colony formation assay. (F) Cell apoptosis was examined using flow cytometry assay in these transfected cells. (G and H) The expression of Bax, Bcl-2 and c-caspase-3 was quantified by Western blot in these transfected cells. (I and J) Cell cycle distribution was checked by flow cytometry assay in these transfected cells. (K and L) Cell migration and invasion were examined using transwell assay in these transfected cells. *P<0.05.