Skip to main content
. 2020 Sep 23;9:e59974. doi: 10.7554/eLife.59974

Figure 2. RRF knockdown creates queues of stalled ribosomes upstream of stop codons and high ribosome density in the 3′-UTR.

Figure 2.

(A) Ribosome density (in reads per million mapped reads) on the rpsB gene 60 min after the 2nd media change in the wild-type (top) and RRF knock-down strain (bottom). (B–D) Average ribosome density aligned at stop codons from standard ribosome profiling after 5 min (B), 15 min (C), and 60 min (D). (E) Ratio of ribosome density (in RPKM) in the 3′-UTR and upstream ORF, with and without high salt in the lysis buffer. (F–H) Average ribosome density at stop codons in libraries prepared with the same biological samples as (B–D) but with high-salt lysis buffer.