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. 2020 Sep 22;9:e55744. doi: 10.7554/eLife.55744

Figure 5. Non-motile variants of P. aeruginosa elicit a reduced activation of the PIP3 pathway in macrophages.

Figure 5.

(A) Dual-seq analysis revealed that the pathways leading to the signaling molecules PI(3,4)P2 and PI(3,4,5)P3 (in red), and the activation of the calcium-dependent cell rearrangements are expressed at lower levels in RAW264.7 cells infected with the flagellar mutants in comparison to PA14. The figure is based on annotations of the KEKK pathway mmu04070. The intersection of three pathways with each top 1000 genes of the motility mutants is shown. (B) Phagocytic uptake of PA14 and its motility variants in RAW264.7 cells 1 hr post infection using an MOI of 1 in the presence (blue, +) or absence (black, −) of 40 µM PI(3,4,5)P3. Mean ± standard deviation of three individual experiments is displayed. *p<0.05; ***p<0.001 (one-way analysis of variance (ANOVA), Dunnett’s post-hoc test).

Figure 5—source data 1. Enrichment of calcium and PIP3-related functions of a downregulated (log2FC < 0) subset of Top 1000 genes from ΔfliC, ΔflgK and ΔmotABCD mutants.