Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Chemical compound, drug | Bacto-Agar | BD | 214010 | - |
| Chemical compound, drug |
Gentamycin | Sigma | G1397-10ml | - |
| Chemical compound, drug | PtdIns(3,4,5)P3 | Echelon | P-3916 | - |
| Chemical compound, drug | Shuttle PIPTM Carrier 2 | Echelon | P-9C2 | - |
| Chemical compound, drug | DMEM | Gibco | 31885–023 | - |
| Chemical compound, drug | RPMI | Gibco | 21875–034 | - |
| Chemical compound, drug | FCS | Biowest | S1810-500 | - |
| Chemical compound, drug | BSA | Sigma | A9418-50 | - |
| Chemical compound, drug | HEPES | Gibco | 15630–056 | - |
| Chemical compound, drug | Cytochalasin D | Sigma | C2618-200UL | - |
| Chemical compound, drug | Spermidine | Sigma | S0266-25G | - |
| Chemical compound, drug | Norspermidine | Sigma | I1006A-100G-A | - |
| Chemical compound, drug | TritonX-100 | BioRad | #161–0407 | - |
| Chemical compound, drug | RNAprotect Bacteria Reagent | Qiagen | #76506 | - |
| Chemical compound, drug | Pyridine, HPLC Grade, 99.5+% | Alfa Aesar | 110-86-1 | - |
| Chemical compound, drug | Phenylisothiocyanate (PITC) | Sigma | 78780 | - |
| Antibody | Rabbit anti- AKT | Cell Signaling Technology | #9272 | 1:1000 |
| Antibody | Rabbit anti-p-AKT (Ser473) | Cell Signaling Technology | #9271 | 1:1000 |
| Antibody | Mouse anti-GAPDH | Calbiochem | CB1001 | 1:10000 |
| Antibody | Goat anti-mouse IgG+IgM (H+L) | Dianova | 115-035-068 | 1:10000 |
| Antibody | Goat anti-rabbit IgG (H+L) | Dianova | 111-035-045 | 1:10000 |
| Commercial assay, kit | NEBNext Single Cell/low input Input RNA Kit | NEB | E6240S | - |
| Commercial assay, kit | Ribo-Zero rRNA removal Kit | Illumina | - | - |
| Commercial assay, kit | NEBNext Ultra II Directional RNA Library Prep Kit | NEB | E7760L | - |
| Commercial assay, kit | ELISA MAX Standard Set Mouse TNF-α | BioLegend | #430901 | - |
| Commercial assay, kit | ELISA MAX Standard Set Mouse IL-6 ELISA MAX Standard Set Mouse IL-6 ELISA MAX Standard Set Mouse IL-6 |
BioLegend | #431301 | - |
| Cell line (M. musculus) | RAW264.7 macrophages | - | RRID:CVCL_0493 | - |
| Cell line (M. musculus) | J774 cells | - | RRID:CVCL_0358 | |
| Cell line (M. musculus) | BMDMs C57BL/6 | Own breeding | N/A | - |
| Cell line (M. musculus) | L929 | - | RRID:CVCL_0462 | - |
| Strain, strain background (P. aeruginosa) | PA14 wild-type | Amiel et al., 2010 | N/A | - |
| Strain, strain background (P. aeruginosa) | ΔfliC | Amiel et al., 2010 | N/A | - |
| Strain, strain background (P. aeruginosa) | ΔflgK | Amiel et al., 2010 | N/A | - |
| Strain, strain background (P. aeruginosa) | ΔmotABCD | Amiel et al., 2010 | N/A | - |
| Strain, strain background (P. aeruginosa) | ΔPA14_63120 | This study | N/A | - |
| Strain, strain background (P. aeruginosa) wild-type | CH2677 | Hornischer et al., 2019 | N/A | Clinical Isolate |
| Strain, strain background (P. aeruginosa) wild-type | CH3290 | Hornischer et al., 2019 | N/A | Clinical Isolate |
| Strain, strain background (P. aeruginosa) | CH3797 | Hornischer et al., 2019 | N/A | Clinical Isolate |
| Strain, strain background (P. aeruginosa) | CH4035 | Hornischer et al., 2019 | N/A | Clinical Isolate |
| Strain, strain background (P. aeruginosa) | CH4433 | Hornischer et al., 2019 | N/A | Clinical Isolate |
| Strain, strain background (P. aeruginosa) | CH4438 | Hornischer et al., 2019 | N/A | Clinical Isolate |
| Strain, strain background (P. aeruginosa) | CH5334 | Hornischer et al., 2019 | N/A | Clinical Isolate |
| Strain, strain background (P. aeruginosa) | CH5528 | Hornischer et al., 2019 | N/A | Clinical Isolate |
| Strain, strain background (M. musculus) | C57BL/6 | Own Breeding | N/A | - |
| Recombinant DNA reagent | pEX18-Gm | Lab stock | N/A | Suicide vector |
| Software, algorithm | ImageJ | - | RRID:SCR_003070 | v1.52p |
| Software, algorithm | Prism | Graphpad | N/A | v5.01 |
| Software, algorithm | Zeiss SEM Smart | Zeiss | N/A | v5.05 |
| Software, algorithm | ITEM Build 1210 | Olympus Soft Imaging | N/A | - |
| Software, algorithm | Skyline | MacCoss Laboratory | N/A | v18.305 |
| Software, algorithm | R Project | - | RRID:SCR_001905 | v3.6.1 |
| Software, algorithm | Analyst | ABSciex | N/A | v1.6.2 |
| Software, algorithm | Fastqc | N/A | RRID:SCR_014583 | v0.11.4 |
| Software, algorithm | tophat2 | N/A | RRID:SCR_013035 | v2.0.12 |
| Software, algorithm | Bowtie2 | N/A | RRID:SCR_005476 | v2.3.4.1 |
| Software, algorithm | Samtools | N/A | RRID:SCR_002105 | v0.1.19.0 |
| Software, algorithm | bedtools | N/A | RRID:SCR_006646 | v2.29.0 |
| Software, algorithm | DAVID (https://david.ncifcrf.gov/) | N/A | RRID:SCR_001881 | v6.7 |
| Software, algorithm | R library Rsubread | N/A | RRID:SCR_016945 | v1.34.7 |
| Software, algorithm | R library edgeR | N/A | RRID:SCR_012802 | v3.24.3 |
| Software, algorithm | R library eulerr | Larsson, 2018 | N/A | v6.0.0 |
| Software, algorithm | R library superheat | Barter and Yu, 2018 | N/A | v0.1.0 |
| Software, algorithm | R library pheatmap | N/A | RRID:SCR_016418 | v1.0.12 |
| Software, algorithm | R library ggplot2 | N/A | RRID:SCR_014601 | v3.1.1 |
| Software, algorithm | R library RColorBrewer | N/A | RRID:SCR_016697 | v1.1–2 |
| Other | Reference genome M. musculus | GRCm38/mm10 | N/A | - |
| Other | Reference genome P. aeruginosa UCBPP-PA14 | NC_008463.1 | N/A | - |