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. 2020 Oct 6;9:e59614. doi: 10.7554/eLife.59614

Figure 1. DMD-4 shows both sexually dimorphic and non-dimorphic expression.

(A) Schematic of the GFP-tagged dmd-4 locus in the dmd-4 (ot935) allele. The DM, DMA, and EH1 domains of DMD-4 are indicated in the colored boxes and labeled above the locus. (B) DMD-4::GFP is expressed in head sensory neurons and pharyngeal cells/the head mesodermal cell (hmc) in both sexes, and the phasmid sensory neurons PHA and PHB in both sexes until adulthood, when it is degraded in the male phasmids. Expression in the pharyngeal cells onsets during the ‘bean’ stage of embryogenesis. GFP is shown as color-inverted black and white. All scale bars (red horizontal bars) indicate 10 microns. Head images are maximum intensity projections of the entire worm; tail images are maximum intensity images of one half of the tail to clearly display phasmid nuclei. (C) Sexually dimorphic connectivity of the PHA and PHB neurons based on electron micrograph reconstruction (Cook et al., 2019; Jarrell et al., 2012).

Figure 1.

Figure 1—figure supplement 1. DMD-4::GFP is dimorphically expressed in the phasmid neurons.

Figure 1—figure supplement 1.

Quantification of the number of phasmid neurons expressing DMD-4::GFP in L1/L4/adult animals of both sexes is shown (maximum is 4 cells for PHAL/R and PHBL/R), where each dot represents the number of neurons expressing in a single animal, blue horizontal bars indicate median, and black boxes indicate quartiles. Representative images are shown in Figure 1B.