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. 2020 Sep 17;9:e55585. doi: 10.7554/eLife.55585

Figure 5. IC-thalamus connectivity.

Figure 5.

(A) Coronal sections depicting the thalamus with subregions that connect to the IC. (B) Representative images from excitatory inputs (top row, eGFP-expressing cell bodies) and outputs (bottom row, eYFP-positive neurons). Different Bregma levels are shown for each IC subregion as indicated on the images. Scale bar = 500 µm. (C) Comparison of inputs to excitatory or inhibitory neurons of all three IC subregions (left) and of outputs from excitatory IC neurons to the thalamus (aIC, red; mIC, green; pIC, blue). Values are calculated as percentage of total cells (RV) or of total pixels (AAV). Data shown as average ± SEM, n = 3 mice per condition. One-way ANOVAs per subregion followed by Tuckey’s multiple comparison test were performed to generate p-values. Significant differences between inputs to excitatory or inhibitory neurons to IC subregions or between outputs from the IC subregions were labeled as **p<0.01, *p<0.05. For detailed statistics see Supplementary file 3. (D) Input cell density (top row) and output density (bottom row) plots along the anterior-posterior axis. Thalamus regions of interest are shown, n = 3 mice per condition, data shown as average ± SEM.