Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) |
Camk2a-Cre | https://www.jax.org/strain/005359 | IMSR Cat# JAX:005359, RRID:IMSR_JAX:005359 |
B6.Cg-Tg(Camk2a-cre) T29-1Stl/J |
| Genetic reagent (M. musculus) |
GAD2-Cre | https://www.jax.org/strain/010802 | IMSR Cat# JAX:010802, RRID:IMSR_JAX:01002 |
Gad2tm2(cre)Zjh/J |
| Software, algorithm | CellProfiler 3.0.0 | https://cellprofiler.org/ | CellProfiler Image Analysis Software, RRID:SCR_007358 |
|
| Software, algorithm | FIJI | Fiji is just ImageJ, NIH (https://imagej.net/Fiji) |
Fiji, RRID:SCR_002285 | |
| Software, algorithm | Autonomous_neuron_ detection.ijm |
This paper, GitHub (https://github.com/GogollaLab/ tracing_quantification_and_analysis/ blob/master/autonomous_ neuron_detection.ijm) |
||
| Software, algorithm | Counting_RV.ijm | This paper, GitHub (https://github.com/GogollaLab/ tracing_quantification_and_analysis/ blob/master/counting_RV.ijm) |
||
| Software, algorithm | Roi_set_atlas | This paper, GitHub (https://github.com/GogollaLab/ tracing_quantification_and_analysis/ tree/master/ROI_set_atlas) |
||
| Software, algorithm | Leica Application Suite X 3.3.0.16799 |
https://www.leica-microsystems.com/
products/microscope-software/ details/product/leica-las-x-ls/ |
Leica Application Suite X, RRID:SCR_013673 |
|
| Software, algorithm | Autonomous_pixel_ detection.ijm |
This paper, GitHub (https://github.com/GogollaLab/ tracing_quantification_and_analysis/ blob/master/autonomous_ pixel_detection.ijm) |
||
| Software, algorithm | Counting_AAV.ijm | This paper, GitHub (https://github.com/GogollaLab/ tracing_quantification_and_analysis/ blob/master/counting_AAV.ijm) |
||
| Software, algorithm | Python 3.6 | http://www.python.org/ | Python Programming Language, RRID:SCR_008394 |
|
| Software, algorithm | Analysis_RV.py | This paper, GitHub (https://github.com/GogollaLab/ tracing_quantification_and_analysis/ blob/master/analysis_RV.py) |
||
| Software, algorithm | Analysis_AAV.py | This paper, GitHub (https://github.com/GogollaLab/ tracing_quantification_and_analysis/ blob/master/analysis_AAV.py) |
||
| Software, algorithm | GraphPad Prism | GraphPad Software, CA (https://graphpad.com) |
GraphPad Prism, RRID:SCR_002798 |
|
| Other | AAV2/5-EF1a-DIO-eYFP | UNC Vector Core https://www.med.unc.edu/ genetherapy/vectorcore/ |
In-Stock AAV Vectors – Dr. Karl Deisseroth, 100 ul Aliquots |
5.6 × 1012 vg/ml |
| Other | AAV2/8-EF1a-FLEX- TVA-mCherry |
UNC Vector Core https://www.med.unc.edu/ genetherapy/vectorcore/ |
In-Stock AAV Vectors – Dr. Karl Deisseroth, 100 ul Aliquots |
4.2 × 1012 vg/ml |
| Other | AAV2/8-CA-FLEX-RG | UNC Vector Core https://www.med.unc.edu/ genetherapy/vectorcore/ |
In-Stock AAV Vectors – Dr. Karl Deisseroth, 100 ul Aliquots |
2.5 × 1012 vg/ml |
| Other | SAD∆G-eGFP(EnvA) | UNC Vector Core https://www.med.unc.edu/ genetherapy/vectorcore/ |
In-Stock AAV Vectors – Dr. Karl Deisseroth, 100 ul Aliquots |
3 × 108 ffu/ml |