Fig. 1.

Phylogenetic tree based on the deduced 424-aa sequence of the complete HE protein of BCoV. Sequence alignments and clustering were performed using ClustalW in MEGA 7.0 software. The tree was constructed by the neighbor-joining method with bootstrap values calculated for 1,000 replicates. The strains in this study are indicated by circles, and the other Chinese BCoV strains are indicated by triangles. The Liaoning strains are indicated in red (red circles and red triangles). The BCoV strain with recombinant HE and the insertion strain with recombinant HE with an insertion are indicated in the figure