. 2020 Jul 15;82(9):1253–1259. doi: 10.1292/jvms.20-0301

Table 1. Details of the non-synonymous single nucleotide polymorphisms (SNPs) analyzed of IL4R and IL13, and in silico prediction of their possible effects.

Gene	Dog chromosome	Reference SNP ID	SNP location	DNA strand	Amino acid substitution	In silico prediction ^a)
Gene	Dog chromosome	Reference SNP ID	SNP location	DNA strand	Amino acid substitution	PolyPhen	SIFT	PROVEAN
IL4R	6	rs9193906	NC_006588.3	Template	p.Thr220Arg	0.45	0.49	−0.673
		rs9193906	g.19263381 G>C	Template	p.Thr220Arg	Benign	Tolerated	Neutral
		rs24378020	NC_006588.3	Template	p.Arg688Cys	0.76	0.06	−0.709
		rs24378020	g.19255617 G>A	Template	p.Arg688Cys	Possibly damaging	Tolerated	Neutral
		rs851400460	NC_006588.3	Template	p.Ser781Leu	0.00	1.00	0.335
		rs851400460	g.19255337 G>A	Template	p.Ser781Leu	Benign	Tolerated	Neutral

IL13	11	rs22147008	NC_006593.3	Coding	p.Thr81Ala	0.99	0.04	−4.86
IL13	11	rs22147008	g.20960082 A>G	Coding	p.Thr81Ala	Probably damaging	Deleterious	Deleterious

a) Bioinformatics tools used to predict the impact of an amino acid substitution on protein function.