Table 2. Summary of the primers, polymerase chain reaction (PCR) conditions, and genotyping methods used for the four single-nucleotide polymorphisms.
| Gene | Polymorphism | Primer pairs | PCR conditions | Restriction enzyme | RFLP a) Genotype | |||
|---|---|---|---|---|---|---|---|---|
| IL4R | rs9193906; G >C | 5′-GGCTCAGACCTACAACAGCA-3′ | 94°C, 30 sec; 57°C, 15 sec; 72°C, 25 sec for 30 cycles | PmlI | C: 259 bp (uncut); G: 210 & 49 bp | |||
| 5′-GCTCATTTGTTACCGCAGCC-3′ | ||||||||
| rs24378020; G >A | 5′-CCCCTGTTCACCTTTGGACT-3′ | 94°C, 30 sec, 60°C, 5 sec; 72°C, 10 sec for 30 cycles | PvuII | G: 309 bp (uncut); A: 191 & 118 bp | ||||
| 5′-TGAGGACCTGTCTCCACAGC-3′ | ||||||||
| rs851400460; G >A | 5′-GTGGCAAGGCCCACATAGT-3′ | 94°C, 30 sec; 60°C, 10 sec; 72°C, 20 sec for 30 cycles | AvaI | A: 311 bp (uncut); G: 160 & 151 bp | ||||
| 5′-CCTGCAGACATCAGCAACCA-3′ | ||||||||
| IL13 | rs22147008; A >G | 5′-TCTCAAACCCCACCTCCTGT-3′ | 94°C, 30 sec; 65°C, 15 sec; 72°C, 25 sec for 30 cycles | PspOMI | A: 230 bp (uncut); G:168 & 62 bp | |||
| 5′-AGGACAGAGGGCCTTACCC-3′ | ||||||||
a) RFLP: restriction fragment length polymorphism.