Table 7.
Effects of dietary linseed oil supplementation, with and without soybean isoflavone, on expression of genes related to lipid metabolism of breast muscle in yellow-feathered chickens
| Genes | 4% LA | 2% LA + 2% LO | 4% LO | 2% LA + 2% LO + SI | 4% LO + SI | SEM | P values1 | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Treatment | LO | SI | LO × SI | |||||||
| FADS1 | 1.03b | 1.15ab | 1.30a | 1.37a | 1.30a | 0.040 | 0.038 | 0.16 | 0.61 | 0.14 |
| FADS2 | 1.04c | 1.32bc | 1.51b | 1.73a | 1.79a | 0.050 | 0.013 | 0.12 | 0.026 | 0.21 |
| ELOVL2 | 1.06b | 1.15b | 1.23ab | 1.98a | 2.03a | 0.111 | 0.011 | 0.16 | 0.053 | 0.28 |
| ELOVL5 | 1.05b | 1.41ab | 1.59a | 1.62a | 1.68a | 0.068 | 0.015 | 0.43 | 0.39 | 0.47 |
| FAS | 1.04 | 0.88 | 0.98 | 0.75 | 0.82 | 0.039 | 0.11 | 0.32 | 0.067 | 0.85 |
| LPL | 1.02c | 1.40a | 1.34ab | 1.10bc | 1.17abc | 0.047 | 0.044 | 0.94 | 0.043 | 0.52 |
| HMGCR | 1.03b | 1.29a | 1.22ab | 1.11ab | 1.31a | 0.035 | 0.019 | 0.35 | 0.60 | 0.060 |
| CPT1α | 1.03b | 2.74a | 2.18a | 2.31a | 2.20a | 0.158 | 0.007 | 0.28 | 0.55 | 0.46 |
| SREBP-1 | 1.02 | 1.28 | 0.98 | 1.26 | 1.28 | 0.065 | 0.39 | 0.50 | 0.30 | 0.35 |
LA = lard; LO = linseed oil; SI = 30 mg/kg soybean isoflavone; FADS1 = Fatty acid desaturase 1; FADS2 = Fatty acid desaturase 2; ELOVL2 = fatty acid elongase 2; ELOVL5 = fatty acid elongase 5; FAS = fatty acid synthase; LPL = lipoprotein lipase; HMGCR = 3-hydroxy-3-methylglutaryl-coenzyme A reductase; CPT1α = carnitine palmitoyltransferase-1α; SREBP-1 = sterol regulatory element binding protein-1.
Treatment, all five diets. LO, dietary LO substituted for half or total LA in the last four diets. SI, dietary linseed oil with or without SI in the last four diets.
Means marked with different letters in rows differ significantly at P < 0.05 (n = 6).