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. 2020 Oct 6;10:16577. doi: 10.1038/s41598-020-73641-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Polymerase reactions with SOF-TP, UTP or UTP + RDV-TP to produce RNA extension products. The sequence of the RNA template-loop-primer used for these polymerase extension reactions is shown at the top of the figure. (a) MALDI-TOF mass spectrum of the unextended RNA. Polymerase extension reactions were performed by incubating (b) SOF-TP, (c) UTP and (d) UTP + RDV-TP with pre-assembled SARS-CoV-2 polymerase (nsp12, nsp7 and nsp8) and the indicated RNA template-loop-primer, followed by detection of reaction products by MALDI-TOF MS. The accuracy for m/z determination is approximately ± 10 Da.