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. 2020 Oct 6;10:16577. doi: 10.1038/s41598-020-73641-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Treatment of the RNA products with exonuclease and then combined for analysis by MALDI-TOF MS to determine relative excision of Sofosbuvir, UMP and Remdesivir. Untreated products (0 min) are shown in (a) for SOF and UMP extended RNA and (c) for SOF and UMP + RDV extended RNA. Exonuclease reactions were performed by incubating the purified RNA products with pre-assembled SARS-CoV-2 exonuclease complex (nsp14 and nsp10). The SOF and UMP extended RNAs were treated with the exonuclease complex for 15 min and then combined for purification followed by MALDI-TOF–MS (b). The insets in (a,b) are an enlargement of the 8200–8300 Da portion of the spectrum. The SOF and UMP + RDV extended RNAs were treated with the exonuclease complex for 5 min or 30 min and combined for purification and MALDI-TOF–MS (d,e, respectively). The signal intensities were normalized to the highest peak within each time series. The accuracy for m/z determination is approximately ± 10 Da.