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. 2020 Oct 6;6:97. doi: 10.1038/s41420-020-00333-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Representative images of F4/80 (red) and Arg1 (green) immunohistochemical staining on day 7 after injury in spinal cord lesion areas. The cavity boundary is indicated by the dashed lines. All cell nuclei were counterstained with DAPI (blue). Scale bar, 200 μm. b, c Quantitative analysis of the number of F4/80⁺ microglia/macrophages and the ratio of Arg1⁺/F4/80⁺ microglia/macrophages in the lesion in (a). d Representative images of F4/80 (red) and iNOS (green) immunohistochemical staining on day 7 after injury in spinal cord lesion areas. The cavity boundary is indicated by the dashed lines. All cell nuclei were counterstained with DAPI (blue). Scale bar, 200 μm. e Quantitative analysis of the ratio of iNOS⁺/F4/80⁺ microglia/macrophages in the lesion in d. LA, lesion core; LA, lesion adjacent. All data are presented as means ± SEM (n = 5 mice per group). *P < 0.05.