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. 2020 Oct 6;11:5010. doi: 10.1038/s41467-020-18852-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Confocal images of several body wall muscle cells immunostained with antibodies to PAT-6 (α-parvin) from wild type and the strain VC20366 identified by screening 574 MMP strains. Arrowheads point to the boundaries between muscle cells. b Schematic representation of domains in C. elegans PIX-1a, and the location and nature of 7 pix-1 mutants and evaluation of their phenotypes. The asterisk denotes the pix-1 mutant allele found in the original strain VC20366. c Muscle-specific expression of a wild-type cDNA for PIX-1 tagged with HA rescues the phenotype of pix-1(gk299374). The transgene is sfEx61[myo-3p::HA-PIX-1; sur-5::nls::GFP], in which sur-5::nls::GFP is the transformation marker showing GFP in nuclei. Note that PAT-6 has been restored to the muscle cell boundaries (indicated by arrowheads), and that HA-tagged PIX-1 localizes to muscle cell boundaries, dense bodies, and M-lines. d Comparison of wild type vs. pix-1(gk299374) immunostained with antibodies to the indicated IAC proteins and imaged by confocal microscopy. Arrowheads denote muscle cell boundaries. Note that all four proteins are present in wild type but missing from muscle cell boundaries in the pix-1 mutant. Each image is a representative image obtained from at least 2 fixation and immunostaining experiments, and imaging at least three different animals. Scale bars in (a), (c) and (d), 10 μm.