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. 2020 Oct 6;11:5010. doi: 10.1038/s41467-020-18852-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Putative PIX-1 biochemical pathway based on what is known of PIX proteins in mammals and other cell types in C. elegans. Also indicated is a summary of the results shown in b. b Confocal images of body wall muscle from the indicated mutants immunostained with anti-PAT-6. Note reduced or disorganized PAT-6 localization at muscle cell boundaries (indicated by arrowheads) in pak-1, git-1, ced-10, and pak-2 mutants, but normal PAT-6 localization in mig-2, rac-2, and max-2 mutants. A higher magnification view (bottom row, right) of pak-2(ok332) is shown because it has a more subtle defect than the other mutants. Results on second alleles for rac-2 and ced-10 are shown in Supplementary Fig 3. Each image is a representative image obtained from at least 2 fixation and immunostaining experiments, and imaging at least three different animals of each strain. Scale bar, 10 μm.