Figure 3.

A NF-κB binding site in the IL-6 promoter is involved in AdV 7-induced IL-6 transactivation. (A) Schematic illustration of all the IL-6 promoter-controlled luciferase reporter gene plasmids. (B) BEAS-2B cells were first transfected with (-1,000/+11)IL-6-Luc, and then either mock-infected or infected with ascendant doses of AdV 7 for 24 h. Afterwards, cells were lysed and luciferase activity was measured. Data shown are mean ± SD of three independent experiments with each condition performed in duplicate. *p < 0.05; **p < 0.01. (C,D) BEAS-2B cells were first infected with either (C) serially truncated IL-6 promoter plasmids or (D) site-directed mutated IL-6 promoter plasmids, and then were either mock-infected or infected with 1 MOI of AdV 7. Twenty-four hours later, cells were lysed, and luciferase activity was measure. Data shown are mean ± SD of three independent experiments with each condition performed in duplicate.