Figure 5.

AdV 7 infection induces IL-6 expression via p38/NF-κB signaling pathway. (A,B) BEAS-2B cells were first (A) transfected with (-1,000/+11)IL-6-Luc and then (A,B) mock-infected or infected with 1 MOI AdV 7 and treated with different signaling pathway inhibitors. Twenty-four hours later, (A) cells were lysed and luciferase activity was measured, or (B) IL-6 concentration in the supernatant was measured by ELISA. Data shown are mean ± SD of three independent experiments with each condition performed in duplicate. *p < 0.05; **p < 0.01; ***p < 0.001. (C,D) BEAS-2B cells were first transfected with control siRNA or p38 siRNA, and then were mock-infected or infected with 1 MOI AdV 7. Twenty-four hours later, (C) IL-6 concentration in the supernatant was measured by ELISA, or (D) cells were lysed and the expression of p38, p-p38, p65, and p-p65 was determined by Western blot. (C) Data shown are mean ± SD of three independent experiments with each condition performed in duplicate. Ns, not statistically significant; *p < 0.05. (D) One representative result out of three is shown.