FIGURE 13.

CD25-IL2 did not effectively control EAE in an IL-2 replete environment. On day 0, C57BL/6 mice were immunized with 200 μg MOG35-55 emulsified in CFA. Mice received 400 ng of Pertussis toxin intraperitoneal on days 0 and 2. At peak disease (day 15) mice were immunized with saline (control group) or with 2 nmoles (A) IL2-CD25 in saline or (B) 2 nmoles CD25-IL2 in saline. Mice were boosted on day 17 with the same formulation. (C) At peak disease (day 16) mice were immunized with 2 nmoles CD25-IL2 in saline or with saline alone. Mice were then boosted on days 18, 20, 22 with the same formulation. Shown are the daily mean clinical EAE scores and daily mean initial weight through the end of the experiment. Error bars represent SEM. Arrows indicate the schedule of FP administration.