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. 2020 Sep 23;11:541619. doi: 10.3389/fimmu.2020.541619

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Copyright © 2020 DeOca, Moorman, Garcia and Mannie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fusion proteins exhibited transient binding to cell-surface transmembrane CD25. HEK cells expressing transmembrane full-length mouse CD25 were incubated with 1 μM (A) or designated concentrations (B, x-axis) of the His-tagged proteins CD25-IL2, IL2-CD25, or IL-2. These cells were extensively washed and were incubated with an APC-labeled anti-Histag mAb. Shown are (A) representative dotplots together with percentages of APC⁺ cells (B, top) and the anti-Histag MFI of GFP⁺ cells (B, bottom). Statistical significance was analyzed by use of a one-way ANOVA with the Holm-Sidak multiple comparisons test. Statistically significant (p < 0.05) differences were noted as indicated (a, 1 vs 2) (b, 1 vs 3). Error bars represent SD. These data represent three independent experiments.