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. 2020 Sep 23;11:522729. doi: 10.3389/fphar.2020.522729

Figure 4.

Figure 4

Lian Hua Qing Wen capsule (LHQW) protected alveolar epithelial cells against TRAIL-induced apoptosis. Lung tissue come from ALI model mice were fixed in 4% PFA, then 3-5-μm sections were prepared after paraffin embedding. All sections were subjected to immunohistochemistry. In the macrophage-epithelial co-cultural model, human pulmonary alveolar epithelial cells (HPA cells) in 6-well plates were incubated with supernatant from THP1 cells that were treated with LHQW, tunicamycin (TM), and lipopolysaccharide (LPS). (A) The effect of LHQW on increasing the cell counting of HPA cells in the macrophage-epithelial co-cultural model, which was based on MTT assays. (B, C) LHQW alleviated apoptosis in the macrophage-epithelial co-cultural model measured by (B) AV-PI assay and (C) caspase3 activity. (D) The number of apoptotic cells (dark brown) in lung tissue measured by TUNEL (200×). THP1 cells in 6-well plates were first treated with PMA for 24 h, followed by LHQW and LPS for another 24 h, then the supernatant was collected. The data was presented as the mean ± S.E.M. of three independent experiments. # P < 0.05 vs. the NC group. * P < 0.05, ** P < 0.01 vs. the LPS group. LHQW-L: 50 μg/ml; LHQW-M: 100 μg/ml; LHQW-H: 500 μg/ml; TM: tunicamycin.