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. 2020 Sep 23;11:579761. doi: 10.3389/fimmu.2020.579761

Figure 4.

Figure 4

Adjuvants differentially influence germinal center kinetics. Mice were immunized subcutaneously with CTH522 protein antigen either alone or in the presence of the indicated adjuvant. (A) Representative plots of antigen-specific germinal center (GC) B cells (B220+CD38-GL7+ cells binding CTH522 coupled to AF488) in the draining inguinal lymph nodes at days 7 and 14 post immunization. Lower panels: percentages (of B220+) and numbers of antigen-specific germinal center B cells. Each group consisted of 2 (antigen alone) or 4 (antigen +adjuvants) mice. Data are representative of two independent experiments. (B) Draining lymph node GC areas (upper panel) and number of GCs as indicated by clusters of Ki67+ cells. Groups consisted of 3 (antigen alone) or 8 (antigen +adjuvants) mice. The experiment was performed once. Each point represents mean+ SEM. Statistically significant differences between the SE and AH groups are indicated by * and *** whilst significant differences between the SE and CAF01 groups are indicated by # and ### (One-way ANOVA with Tukey's correction for multiple group comparison, using the SE group as reference and significance levels of p < 0.05 and p < 0.001, respectively).