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. 2020 Sep 23;14:547215. doi: 10.3389/fncel.2020.547215

Figure 5.

Figure 5

Effect of D-BHB on autophagy-related proteins in the parietal cortex 24 h after the hypoglycemic coma. Changes in (A) LC3-II/Actin and SQSTM1/p62/Actin, (B) BECN1/Actin, ATG7/Actin, and ATG12–ATG5/Actin. (C) Confocal representative images of immunoreactivity to LC3 and SQSTM1/p62 in animals treated and non-treated with D-BHB (500 mg/kg). Orthogonal images (x-z upper) and y-z (right) orientations are shown. Data are expressed as mean ± SEM. Statistical analysis was performed by one-way ANOVA followed by the Fisher post hoc test for multiple comparisons. *p ≤ 0.05 vs. control, #p < 0.05 vs. coma, n = 4–5 control, n = 5–6 Coma, n = 5 Coma + BHB. Scale bar = 10 μm.