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. 2020 Sep 23;14:547215. doi: 10.3389/fncel.2020.547215

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Copyright © 2020 Torres-Esquivel, Montiel, Flores-Méndez and Massieu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of D-BHB on autophagy-related proteins in the hippocampus 24 h after the hypoglycemic coma. (A) Changes in LC3-II/Actin and SQSTM1/p62/Actin, (B) BECN1/Actin, ATG7/Actin, and ATG12–ATG5. (C) Confocal representative images of immunoreactivity to LC3 and SQSTM1/p62 in animals treated and non-treated with D-BHB (500 mg/kg). Orthogonal images (x-z upper) and y-z (right) orientations are shown. Data are expressed as mean ± SEM. Statistical analysis was performed by one-way ANOVA followed by the Fisher post hoc test for multiple comparisons. *p ≤ 0.05 relative to control, ^#p < 0.05 relative to coma, n = 4–5 control, n = 5–6 Coma, n = 5 Coma + BHB. Scale bar = 10 μm.