FIGURE 6.

MALT1 inhibition abolished Notch1-induced NF-κB activation. (A) NF-κB reporter assays were performed in CCRF and MOLT-4 cells treated with indicated concentrations of MI-2. (B) Exogenous Notch1 significantly upregulated tNF-κB activity. NF-κB reporter assays were performed in CCRF and MOLT-4 stably expressing Notch1-IC treated with indicated concentrations of MI-2. The Notch1-IC-induced luciferase activity data were normalized to empty vector-transfected cells. (C) NF-κB reporter assays were conducted in 293T stably expressing Notch1-IC. Cells were treated with indicated concentrations of MI-2. The Notch1-IC-induced luciferase activity data were normalized to empty vector-transfected 293T cells. (D) The effect of MI-2 on IκBα expression in CCRF and MOLT-4 is shown. (E) Nuclear and cytoplasmic expression of NF-κB subunits p65 were assessed by western blot following exposure to MI-2 and TNF-α. *P < 0.05, **P < 0.01.