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. 2020 Sep 23;10:539805. doi: 10.3389/fonc.2020.539805

FIGURE 3.

FIGURE 3

Recruitment effect of lncRNA BLACAT1 on EZH2 to regulate the function of PC cells. (A) EZH2 gene expression in PC on the basis of TCGA, the abscissa represents sample type, the ordinate represents expression value, the red box represents normal sample, and the gray box represents tumor sample. (B) Intersection of the results of lncRNA prediction in the upstream of EZH2 with the differential expressed gene of GSE16515 chip. The middle part represents the intersection of the two sets of data. (C) Differential expression of BLACAT1 in GSE16515 chip, the abscissa represents the sample type, the ordinate represents the expression, the red box represents the tumor sample, the gray box represents the normal sample, and the upper left is the corrected difference P-value. (D) Detection of BLACAT1 expression in PC tissues and paracancerous tissues by qRT-PCR. (E) Subcellular localization of BLACAT1 by FISH assay. (F) RNA pull down experiment to observe whether BLACAT1 can bind to EZH2. (G) Detection of the binding between BLACAT1 and EZH2 by RIP assay. Data were expressed as mean ± SD, unpaired Student’s t-test, n = 3, *P < 0.05.