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. 2020 Sep 23;10:539805. doi: 10.3389/fonc.2020.539805

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Copyright © 2020 Zhou, Gao, Hua and Ji.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of interference with BLACAT1 to promote the expression of CDKN1C on the proliferation, migration and aerobic glycolysis of PC cells, and on the recovery of mitochondrial oxidative phosphorylation by stimulating the expression of CDKN1C. (A) Detection of CDKN1C expression by qRT-PCR. (B) Detection of proliferation of PC cells by EdU test. (C) Detection of the migration of PC cells by Transwell assay. (D) Detection of the invasion of PC cells by Transwell assay. (E) Detection of glucose consumption in PC cells by hexokinase method. (F) Detection of lactate production in PC cells by hexokinase method. (G) Proportion of glycolysis pathways in glucose metabolism. (H) Detection of the expression of key proteins of mitochondrial complex in each group by western blot. (I) Detection of ATP in supernatant of PC cells with ATP Kit. Data were expressed as mean ± SD, unpaired Student’s t-test, n = 3, *compared with sh-BLACAT1 + sh-NC group, P < 0.05.