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. 2020 Sep 23;11:575772. doi: 10.3389/fphar.2020.575772

Table 2.

Primers used for real time PCR (RT-PCR).

Gene name Gene number Primer sequence
GAPDH NM-002046.7 Forward 5’-ACCCAGAAGACTGTGGATGG-3’
Reverse 5’-TCAGCTCAGGGATGACCTTG-3’
SREBP-1C NM-004176.4 Forward 5’-GAGCTCAAGGATCTGGTGGT-3’
Reverse 5’-CAGTGCGCAGACTTAGGTTC-3’
FASN NM-004104.5 Forward 5’-GGCTGCCTACTACATCGACT-3’
Reverse 5’-CGAACAGGAAGAGGCTGTTG-3’
NF-κB NM-003998.4 Forward 5’-AGCAAATAGACGAGCTCCGA-3’
Reverse 5’-TCGGTAAAGCTGAGTTTGCG-3’
ACC NM-198836.2 Forward 5’-CATGAAGGCTGTGGTGATGGA-3’
Reverse 5’-CTTGGTGACTTGAGCGTGAG-3’
PPARα NM_005036.6 Forward 5’-TGCTACTTACCAGCCGCATA-3’
Reverse 5’-GTCTTAGCTGGGTGCATTGG-3’
TNF-α NM_000594.4 Forward 5’-TCAATCGGCCCGACTATCTC-3’
Reverse 5’- ATGTTCGTCCTCCTCACAGG-3’
Nrf2 NM_006164.5 Forward 5’-CGCAGACATTCCCGTTTGTA-3’
Reverse 5’- AGCAATGAAGACTGGGCTCT-3’