Fig. 2.

Effect of HSA-Trx on urban aerosol-dependent inflammatory responses. Male ICR mice were intravenously administered with HSA-Trx (3.5 nmol protein/mouse in sterile saline) or sterile saline alone, immediately before and 24 h after intratracheal administration of urban aerosol particle suspensions administration (200 μg/mouse) or 0.5% methylcellulose solution alone (Cont). Total RNA was extracted from the lungs 48 h after urban aerosol particle suspensions administration and subjected to real-time RT-PCR using a specific primer set for each gene. Values were normalized to Gapdh and are expressed relative to the Control. Values are the mean ± S.E.M.; ^#p < 0.05; ∗∗ or ^##p < 0.01 (∗, vs Control; #, vs UA).