Fig. 3.

Effect of HSA-Trx on urban aerosol-induced ROS production. Male ICR mice were intravenously administered with HSA-Trx (3.5 nmol protein/mouse in sterile saline) or sterile saline alone, immediately before and 24 h after intratracheal administration of urban aerosol particle suspensions administration (200 μg/mouse) or 0.5% methylcellulose solution alone (Cont). Luminescent probe (L-012, 75 mg/kg) was administered 24.5 h after the urban aerosol particle suspensions administration. (A) Isolated lungs were imaged using a FUSION chemiluminescence imaging system. (B) The summed pixel intensity of the ROS signal was determined using standard software for FUSION. (C, D) RAW264 cells were pre-cultured with DCFHDA (100 μM). The cells were then treated with urban aerosols (3.8–60 ng/cm²) (C) or HSA-Trx (0.3–2.5 μM) prior to the addition of urban aerosols (30 ng/cm²) (D) to the medium. After 1 h, the ROS levels were measured using a microplate reader. Values represent the mean ± S.E.M. ^#p < 0.05; ∗∗ or ^##p < 0.01 (B: ∗ vs Control; #, vs UA, C, D: ∗ vs Control, # vs UA (30 ng/cm²)).