Fig. 4.

Effect of HSA-Trx on urban aerosol-induced neutrophil extracellular trap formation. Male ICR mice were intravenously administered with HSA-Trx (3.5 nmol protein/mouse in sterile saline) or sterile saline alone, immediately before and 24 h after intratracheal administration of urban aerosol particle suspensions administration (200 μg/mouse) or 0.5% methylcellulose solution alone (Cont). BALFs were prepared 48 h after the intratracheal administration. (A) The amount of double-stranded DNA (dsDNA) present in the BALF was determined using the Quant-iT™ PicoGreen® dsDNA Assay Kit in accordance with the manufacturer’s protocol. (B) BALF samples (2 μl) were analyzed by immunoblotting with an antibody against citrullinated histone H3 (Cit-H3) or neutrophil elastase. (C) The intensities of these bands were determined using Image J software. n.d. indicates not detected. Values are the mean ± S.E.M.; ^#p < 0.05; ∗∗ or ^##p < 0.01 (∗, vs Control; #, vs UA).