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. 2020 Oct 6;10:16655. doi: 10.1038/s41598-020-73186-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in NF-κB expression in response to different lncRNA-AK149641 levels. (A–D) Western blots showing the expression of the MyD88 and p-NF-κB p65 proteins. p-NF-κB p65 decreased in the lncRNA-AK149641 downregulated group after LPS stimulation. There was no significant difference in the expression of MyD88. (E) The EMSA shows that downregulation of lncRNA-AK149641 inhibits the expression of NF-κB (NSB: Non-Specific Binding; FB: Free Biotin Labeled Probe) (*P < 0.05, n = 3).