Figure 4.

Evolutionary relationships of MhMYBs. Full-length amino acid sequences of R2R3-MYBs from the chrysanthemum transcriptome dataset and arabidopsis genome were first aligned using ClustalW in MEGA6. The phylogenetic tree was constructed according to the neighbor-joining method. Branches corresponding to partitions reproduced in less than 50% of the bootstrap replicates were collapsed. The evolutionary distances were computed using the p-distance method. Thirty-two MhMYBs marked red triangle were identified from the RNA sequencing data of M. halliana. The arabic numerals on the cycle indicated gene families.