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. 2020 Sep 28;2020:6319395. doi: 10.1155/2020/6319395

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Copyright © 2020 Liangkun Xie et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Evaluation of osteogenic differentiation of DPSCs after exosome treatment. (a) PKH67-stained exosomes were phagocytosed by DPSCs (green); no PKH67-stained signal was found in the negative control; nucleus was stained by DAPI (blue). (b) ALP assay of osteogenesis induction medium groups (PC) and exosome-treated group (EX0 and EX7) cultured at days 0 (D0), 7 (D7), and 14 (D14). (c) The expression levels of RUNX2, col-1, and OCN before and after exosome induction were detected by qRT-PCR. (d) Alizarin red staining of PC group and exosome-treated groups (EX0 and EX7) cultured at days 0 (D0), 7 (D7), 14 (D14), and 21 (D21). NC was the negative control. ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001 indicated significant differences.