Figure 5.

SC‐MIC determination after 1 h culture of P. aeruginosa in blood. a) Bacterial purification protocol for bacteria in blood for rapid AST by SRS imaging of D₂O metabolic incorporation. b) SRS images at C–D, off‐resonance (2407 cm⁻¹), and C–H of bacteria in blood after 1 h culture in D₂O containing medium. c) SRS and corresponding transmission images of P. aeruginosa in blood after 1 h culture in D₂O‐containing medium with the addition of serially diluted gentamicin. d) Statistical analysis of C–D intensity in bacteria in (c). The colored points under different concentration stand for different individual bacterium. The dotted lines indicate the cutoff value at 60% of the control sample. The C–D intensities are normalized to the mean of control without antibiotic treatment. Number of cells N ≥ 10 per group. Error bars represent the SEM. Scale bar: 10 µm. e) Comparison of SC‐MIC and susceptibility category for P. aeruginosa isolate and P. aeruginosa in blood. S: sensitive.