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. 2020 Aug 7;7(19):2001334. doi: 10.1002/advs.202001334

Figure 3.

Figure 3

The quantitation of the immunofluorescence staining, gene expression and ALP activity. The relative fluorescence intensity (RFU) of the a) Col‐1 and b) OPN marker of the immunofluorescence staining. c) Real‐Time PCR analysis of the osteogenic markers (COL‐1, Runx2, ALP, and OPN) showing the osteogenesis of hMSCs induced by the osteogenic exosomes at the gene level. Each gene expression was compared with that of the EXO‐D4 treated group. d) ALP activity of osteogenesis of hMSCs induced by the osteogenic exosomes. Each ALP activity was compared with that of the EXO‐D0 treated group. e) The Alizarin Red intensity after all kinds of exosome induction. Each exosome treated group was compared with the EXO‐D0 treated group. *p < 0.05; **p < 0.01, N = 3.