Batf3 expression by CD8 T cells is important for recall response but dispensable for their function. Mice outlined in Fig. 2A were challenged with recall infection at 30 dpi and the donor OT-I cells were analyzed at 6 dpr. (A) The percentage and number of WT and Batf3−/− OT-I cells in the blood at 6 dpr. (B) The corresponding number of WT and Batf3−/− OT-I cells in the blood at 30 dpi and 6 dpr. (C) The percentage and number of WT and Batf3−/− OT-I cells in the IEL compartment at 6 dpr. (D) The granzyme B expression by WT and Batf3−/− OT-I cells in the blood at 6 dpr. (E) The granzyme B expression by WT and Batf3−/− OT-I cells in the IEL compartment at 6 dpr. (F) The IFNγ and TNFα expression by WT and Batf3−/− OT-I cells in the IEL compartment at 6 dpr. The data are pooled from 2 independent experiments with n=7. The data in (A, C, F) are shown as mean ± SEM. In (B, D, E), each dot represents an individual animal.