Figure 2.

Stability of glycosylation features under acidic conditions (100 mM FA). All of the features are highly stable under conditions that may accidentally occur during sample processing. (A) Sialylation decreases only if IVIg is stressed at a high temperature. (B) Galactosylation decreases only very slightly even under the most extreme conditions (37 °C, 2 weeks). (C) Bisection does not change even under severe acidic stress. (D) Fucosylation apparently decreases very slightly due to minor interdependencies with sialic acids. Actual fucosylation is unaffected (Figure S6). * Changes in the respective glycosylation feature compared to the reference condition (−80 °C).