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To find the appropriate dosage of transfected miRNA and assessment time, three dosages of 5, 7.5, and 10 nmol (a) and three times intervals of 24, 48, 72 h (b) were assessed. The results showed that 5 nmol in the 24 h was the most effective dosage in the most effective time (P < 0.0001). The data represent mean ± SD (n = 3), **** P < 0.0001.
